FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91711
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCCGCACGAAA2030.22134749375756452No Hit
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA1570.17118993359575188No Hit
ATCCTAAGGTGAGCGAGTGAACTCTCGTTAAGGAACTCGGCAAAATGACC1130.12321313691923542No Hit
CGTTGAGACAGTGCCCAAATCGTTACGCCTTTCGTGCGGGTCGGAACTTA990.10794779252216201No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCTC150.002214045570.0343718
GAATCAA150.002218843469.99618552
TTAGGAT407.4111813E-752.4971353
TACTTTG200.006933251452.4971358
TCCTAGG200.006933251452.4971352
CTTGGCT200.006933251452.4971354
TCTAAAC200.006933251452.4971353
ATCTTGG200.006933251452.4971357
ACTCCCG200.006933251452.49713540
GTCGGGC200.006933251452.49713557
GGCTAGG200.006933251452.4971356
ATCTAAA305.852563E-446.6641242
ATCTGCT305.852563E-446.6641247
TAGGATT503.4471468E-641.9977074
GGGTCCC350.001246720340.01963816
TGATCTT350.001246720340.01963818
ATCACCA350.001250071839.99781868
CAGGAGG350.001250071839.99781841
AGGATTC459.138761E-538.886775
GTCCCCG400.00240208435.0171818
Produced by FastQC (version 0.11.2)