FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337217
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8020.23782905369539495No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG6160.1826716921151662No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5850.1734787985184614No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5470.16221009023862973No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5260.15598264618924906No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4910.1456035727736146No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4850.14382430304522015No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4620.13700376908637465No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4010.11891452684769747No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3970.11772834702876783No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3850.11416980757197888No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT3750.11120435802465475No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG3680.10912854334152786No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA3600.10675618370366854No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC3570.10586654883947132No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3510.10408727911107685No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT3380.10023219469955547No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGTA305.8735244E-446.666575
TAGGGCT350.001254520639.9999164
ACTGTGC601.214021E-534.9999288
ATTCATA400.00241704334.9999242
TGGTTAA450.00430423231.11104425
TATGCTT1551.8189894E-1229.3547765
CTGTGCG604.9769774E-429.1666059
CGACCGT500.00720333427.999946
ATCTGGG2500.027.999947
TCTAGCC755.5766177E-527.9999398
ACACCTC657.9477124E-426.923026
ATCTAGC1207.377457E-826.2499457
TTTCGGA959.361538E-625.7932421
TCTAACA700.001224772824.99994713
CTCTATG700.001224772824.9999472
ATATGCT1851.4551915E-1124.5945424
GTATGTA1151.4926209E-624.34777516
TTGTCTC1902.1827873E-1123.94731710
ATCTTTT2800.023.749957
AGAAACG2950.023.72876467
Produced by FastQC (version 0.11.2)