FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163871
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT2280.13913383087916714No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2130.12998028937395878No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1940.11838580346736152No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT1860.11350391466458372No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATAT200.006910972852.5584341
CTGTATA200.006910972852.5584341
GTACCCA200.006936067752.51030326
GTTTACG200.006936067752.51030316
AAATGTG200.006936067752.5103035
TCAGACC200.006944447852.4942850
CGTATGC608.367351E-1152.49427841
GTATGCC608.367351E-1152.49427842
AACAGTG305.867727E-446.661587
GAGGCAC305.867727E-446.6615837
CTCGTAT703.8016879E-1044.995139
AAAAAGG757.4942363E-1041.99542266
TGTACAC350.001251411340.007855
ACCTAAA350.001253291839.99564445
AGGCACT350.001253291839.99564438
TACCTAA350.001253291839.99564444
TCGTATG901.03682396E-1038.8846540
TAGGTTG556.655144E-638.1893125
TCTATGC556.66911E-638.1776588
TGAAAAA852.5538611E-937.05478759
Produced by FastQC (version 0.11.2)