FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104836
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2990.2852073715136022No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2020.1926819031630356No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1920.1831431950856576No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1920.1831431950856576No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1790.1707428745850662No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1650.157388683276737No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1620.1545270708535236No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1610.1535732000457858No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT1560.14880384600709678No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1290.1230493341981762No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1270.1211415925827006No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC1190.1135106261207982No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA1170.1116028845053226No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1150.109695142889847No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1140.1087412720821092No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1130.1077874012743714No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA1090.10397191804342021No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1080.10301804723568239No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC1070.1020641764279446No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCC150.002219671269.996663
CTCGTAT200.006935816752.49749839
CTAGTGG200.006935816752.4974983
ATATAAG701.9004801E-839.998092
TATAAGA701.9004801E-839.998093
GGTCCAA459.147353E-538.8870311
ACGGTCC459.147353E-538.887039
ACCGGTA654.8157926E-737.69050615
ATAAGAA753.4913683E-837.331554
CCTAGGG400.00241001234.9983332
TAATACG708.603256E-734.9983320
TCTTACG501.7003951E-434.998332
AAGAATT501.7003951E-434.9983316
CATACAT708.603256E-734.9983331
TCCAAGA501.7003951E-434.9983313
AAGATTA601.2064429E-534.9983318
GTCTTAC601.2064429E-534.998331
TAATGCC708.603256E-734.9983344
ATACATA708.603256E-734.9983332
AAAGATT601.2064429E-534.9983317
Produced by FastQC (version 0.11.2)