FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190720
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT3560.18666107382550337No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2810.14733640939597314No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2700.14156879194630873No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2520.13213087248322147No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2510.13160654362416108No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2400.12583892617449663No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2330.12216862416107382No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2290.12007130872483222No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2210.115876677852349No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT305.8609416E-446.67645619
CGTATTC350.001253460239.99790621
CTTGCTA350.001253460239.99790669
CCTTGCT350.001253460239.99790668
TATTCAC400.00241190935.00734323
ACGTATT400.00241190935.00734320
TACTAAT806.207483E-834.9981657
GTAAATT957.557901E-933.16485224
CGGGAAC552.980494E-431.8248615
CGTAAAT1001.2461896E-831.50660723
AACTAGA450.00429512731.11764115
AAATAAC901.7465027E-731.1094863
GTCTATA450.004300644631.109482
GTTAGAG901.7465027E-731.1094862
ACCAAAG450.004300644631.1094869
TTTTGTA450.004300644631.1094844
TCTAGGG802.4553865E-630.6233942
TAAATTA1052.0037987E-830.00629425
TTACCCA1052.0037987E-830.00629429
ATCTAGC703.476999E-529.9984287