FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273704
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT6380.23309852979861456No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5740.2097156051793178No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC5460.19948557565837546No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5120.18706339695437407No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC3820.13956683132142753No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC3110.1136263993218952Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGAC252.388086E-455.9986843
TATGAAA200.00694705452.4987642
TAGCACC352.0835592E-549.9988254
GTATGCC1450.045.8609942
CTCGTAT1600.041.56152339
TCGTATG1600.041.56152340
TTGAAAA1800.040.83237558
TTGAATC350.001254238239.9990628
CTAGATA459.184808E-538.8879784
AAAAAAG1850.037.83695265
GTCTTCT1850.037.83695249
TGCTTGA1800.036.94357755
TTCTGCT1800.036.94357752
GCCGTCT1800.036.94357746
AAAAAGG1900.036.8412466
CGTATGC1850.035.94510341
GAAAAAA1850.035.94510360
TTAGCAC501.707323E-434.9991763
TCCTACA400.00241650434.9991762
CTTGAAA1950.034.1017657