Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s60.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 273704 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 57 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT | 638 | 0.23309852979861456 | No Hit |
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 574 | 0.2097156051793178 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 546 | 0.19948557565837546 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 512 | 0.18706339695437407 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 382 | 0.13956683132142753 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 311 | 0.1136263993218952 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CATAGAC | 25 | 2.388086E-4 | 55.998684 | 3 |
TATGAAA | 20 | 0.006947054 | 52.498764 | 2 |
TAGCACC | 35 | 2.0835592E-5 | 49.998825 | 4 |
GTATGCC | 145 | 0.0 | 45.86099 | 42 |
CTCGTAT | 160 | 0.0 | 41.561523 | 39 |
TCGTATG | 160 | 0.0 | 41.561523 | 40 |
TTGAAAA | 180 | 0.0 | 40.832375 | 58 |
TTGAATC | 35 | 0.0012542382 | 39.99906 | 28 |
CTAGATA | 45 | 9.184808E-5 | 38.887978 | 4 |
AAAAAAG | 185 | 0.0 | 37.836952 | 65 |
GTCTTCT | 185 | 0.0 | 37.836952 | 49 |
TGCTTGA | 180 | 0.0 | 36.943577 | 55 |
TTCTGCT | 180 | 0.0 | 36.943577 | 52 |
GCCGTCT | 180 | 0.0 | 36.943577 | 46 |
AAAAAGG | 190 | 0.0 | 36.84124 | 66 |
CGTATGC | 185 | 0.0 | 35.945103 | 41 |
GAAAAAA | 185 | 0.0 | 35.945103 | 60 |
TTAGCAC | 50 | 1.707323E-4 | 34.999176 | 3 |
TCCTACA | 40 | 0.002416504 | 34.999176 | 2 |
CTTGAAA | 195 | 0.0 | 34.10176 | 57 |