FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences674320
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC39580.5869616799145806No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC37870.5616027998576344No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC35600.5279392573258987No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC29060.43095266342389366No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15640.23193735911733304Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA14500.21503143907936886Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC10290.15259817297425554No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG8370.12412504448926327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGAGT200.006949777252.50194525
TTAGATA552.9915015E-431.8193634
GTAAACC450.004306430531.11226326
GGTAACT450.00430799331.10995549
CTATAAC604.9832155E-429.16558654
TTTCGGA1352.582965E-1028.5238061
TATACGT906.1688224E-627.223234
CTATCGC657.957628E-426.922089
CAGAGTG808.66327E-526.25097326
GTGTAGG1508.785719E-1025.6714231
TCTGTAT2200.025.4554927
TAGAGTT1451.726039E-824.13882430
TTCGGAA1601.8608262E-924.0633932
GATTCTG901.92627E-423.33592818
TTATCTA1502.4736437E-823.33419825
CTACACT1202.1660326E-623.3341984
ATGTTAT1502.4736437E-823.33419822
ATACGTC1052.038423E-523.3341965
AGTTATA901.9282072E-423.33246640
ACGTATC750.001832644623.33246644
Produced by FastQC (version 0.11.2)