FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93821
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2230.2376866586371921No Hit
TCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCT1110.11831039959070996No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATGGG200.00692055352.522412
TAATTTT200.00692055352.522422
ATAGGAC200.006935171352.4944043
CTATAGG200.006935171352.4944041
GTTAAGT200.006935171352.4944042
ATCTTAC200.006935171352.4944047
CTTAGCT200.006935171352.49440447
TCTGGCG200.006935171352.4944048
TCTAGGT305.8393733E-446.6865813
ATTCCAC305.854756E-446.6616946
ATCTTCC305.854756E-446.6616947
CAGATGC350.001247260840.01706717
AACGTAT350.001250538539.99573519
CCAATCT350.001250538539.9957353
CTAGTAG350.001250538539.9957353
AATCTAT350.001250538539.9957355
CTATTGC350.001250538539.9957358
ATGTCGG459.143168E-538.88474734
CGATCTT2050.037.556975
GATCTTC753.486457E-837.3293536
Produced by FastQC (version 0.11.2)