H3W35AFXY_n01_adr3wtzt14s57.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s57.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99535
Sequences flagged as poor quality	0
Sequence length	76
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT	258	0.25920530466670016	No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG	203	0.20394835987341134	No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA	188	0.18887828402069626	No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA	174	0.17481287989149547	No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC	157	0.15773346059175164	No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT	154	0.1547194454212086	No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC	142	0.14266338473903653	No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA	141	0.1416587130155222	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGT	132	0.1326166675038931	No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC	131	0.13161199578037877	No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT	120	0.120560606821721	No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA	118	0.11855126337469231	No Hit
TTCTAGAGCTAATACATGCAATTAAAACATGAACCTTATGGGACATGTGC	112	0.11252323303360626	No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG	112	0.11252323303360626	No Hit
GTACTACCCGTATGAAGCACAAGTTCAACTACGAACGTTTTAACCGCAAC	108	0.10850454613954891	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	107	0.10749987441603455	No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG	106	0.10649520269252022	No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA	103	0.1034811875219772	No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA	102	0.10247651579846286	No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT	102	0.10247651579846286	No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT	101	0.1014718440749485	No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	100	0.10046717235143417	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	25	2.380401E-4	56.000004	1
TGGGTTG	20	0.006933482	52.5	5
TAGCAGT	20	0.006933482	52.5	69
TTGGGGT	20	0.006933482	52.5	4
TCCTACA	35	2.073625E-5	50.0	2
CCCTAGT	40	4.564466E-5	43.75	1
CCTAGTT	35	0.0012502496	40.0	2
ATCTGGG	80	1.3969839E-9	39.375	7
CTCGTAT	45	9.141439E-5	38.88889	39
GTCTTCT	45	9.141439E-5	38.88889	49
GGTTAAA	55	6.6377597E-6	38.181816	17
GCGGTTA	50	1.6993009E-4	35.000004	15
AAAGGGG	50	1.6993009E-4	35.000004	68
TACCGGT	50	1.6993009E-4	35.000004	30
TATGCCG	60	1.20543555E-5	35.0	43
ATACGTC	40	0.0024088637	35.0	5
AAAGCGA	40	0.0024088637	35.0	62
TGTTGCG	65	2.0848858E-5	32.307693	11
GTTAAAA	65	2.0848858E-5	32.307693	18
CAGGCGC	65	2.0848858E-5	32.307693	17

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content