FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279861
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT8620.3080100478451803No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2950.10540947113031113No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATTA150.002223321269.99856667
TAGTTAT200.006947130452.49892821
ATCTAGG552.2773747E-950.908057
TATTAGC802.910383E-1143.7491072
ATAATAT350.00125426839.9991843
GTATTAG901.03682396E-1038.8950461
TCTAGGC400.002416559334.9992878
ATACCTA400.002416559334.99928769
CTCGTAT1201.8189894E-1234.99928339
GCACAAC601.2134362E-534.99928331
GCCGTCT1201.8189894E-1234.99928346
ATCTGTG904.485628E-934.9992837
GATCTAG2050.034.1456456
TCGTATG1305.456968E-1232.30703440
GATCTTT3250.031.2301316
GTATGCC1357.2759576E-1231.11047642
TCTGTGT450.004303377631.1104768
GAACGTT450.004303377631.11047619
TTTCGGA1850.030.275061
AAAAAAG1401.2732926E-1129.99938865
Produced by FastQC (version 0.11.2)