FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94178
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT1900.20174563061436854No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1420.15077831340652806No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1060.11255282550064771No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC980.10405827263267431No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGAC150.002218548470.03
CCTTATA150.002218548470.02
CACGAGC150.002218548470.033
CTGCGTT150.002218548470.09
TTGCGAC150.002218548470.047
GCATATT150.002218548470.01
ATTAGTG200.00693232952.53
TAGTTTG200.00693232952.54
AAGATTG200.00693232952.543
GCGTAAA200.00693232952.522
TCTGAGC350.001249906840.08
GCGCGTA350.001249906840.020
CTGACAT350.001249906840.058
GTCTTCT350.001249906840.049
CACAACA350.001249906840.062
AAAGGGT350.001249906840.030
TAAAGGG400.002408207635.029
CATGAGT400.002408207635.062
GCTGACA400.002408207635.057
TGACATG400.002408207635.059