FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150105
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2760.18387129009693215No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2430.16188667932447287No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2260.15056127377502415No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2190.14589787148995703No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT2030.13523866626694647No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA1710.11392025582092535No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1680.11192165484161087No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1600.10659205223010558No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1530.10192864994503847No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1510.10059624929216215No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCAC200.00694124252.4982510
TATGTGC200.00694124252.498256
CGTCATG350.0012524939.9986652
GCCCTAG459.165515E-538.88759211
GCAACTG400.002409212835.01049427
TAGTTCT501.703755E-434.99883315
TTGTCTC652.0924093E-532.30661810
GGGAATA450.00429735231.1100751
AGTACTA450.00429735231.1100755
TGACTGG450.00429735231.11007532
AGTTCTA604.9648876E-429.16569516
ACCCGGG604.9648876E-429.16569512
CTATCCC500.007191894627.9990674
TTTCGGA500.007191894627.9990671
CGTTGCA500.007191894627.99906765
TAGTACT500.007191894627.9990674
CTTACAC500.007191894627.9990673
AGGTCCG500.007191894627.99906739
ATATGCT755.5574994E-527.9990654
ATCTTTT1154.8466973E-827.3903927