FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99665
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT3060.3070285456278533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC200.0069337152.49962218
GGTTGCA200.0069337152.49962221
TTACACA200.0069337152.4996224
ATCTAGG404.5647103E-543.7496877
TGCTTGA602.5681948E-740.83304255
AAAAAAG602.5681948E-740.83304265
GTATGCC654.8110815E-737.6920442
TCGTATG654.8110815E-737.6920440
CGTATGC708.5948705E-734.9997541
CTCGTAT708.5948705E-734.9997539
TATGCCG708.5948705E-734.9997543
GCTTGAA708.5948705E-734.9997556
TGGGCCG400.002408966434.999755
TTGCAAA400.002408966434.9997523
GTTATCG400.002408966434.9997512
CTTACAC400.002408966434.999753
ATCTTTT1750.033.99975612
CTTTTTT1800.033.0553214
TCTTTTT1800.033.0553213
GCCGTCT751.4738871E-632.6664346
Produced by FastQC (version 0.11.2)