FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517983
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC33170.6403685063023303No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC31140.6011780309392393No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC30240.5838029433398393No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC24720.47723573939685277No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC13000.25097348754688864Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA12580.2428651133338353Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC8140.15714801451012872No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG7340.14170349219955095No Hit
GTGCAGGGAGACGTGTGCTCTTCCGATCTGTGCAGGGAGACGTGTGCTCT5700.11004222146286655Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGA5530.10676026047186876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA1500.042.0104871
TTCGGAA1750.036.0020332
CTTGTTC450.004305106631.112873
TGGAGTG450.004305106631.112875
CTCTATA450.004305106631.1128728
ATACAGA1301.6552804E-1029.617066
TTGTTCC500.0072047828.0015854
GGCGAGT500.007208176427.9988838
ACGAGTT500.007208176427.9988869
GAGACGA3500.027.99887866
CTTTTTT2600.025.5759019
TGGGCGG851.3080187E-424.70489510
GACGAGT2500.023.79904768
CTAGACT750.001831075323.3346524
ACGAGTG1953.092282E-1123.332469
CTAATCT5200.022.88370170
GACACTC35200.022.86840270
CGAAATT1102.9181843E-522.27398513
TCTTTTT3300.022.2718378
CATAGCA952.7774865E-422.1065124
Produced by FastQC (version 0.11.2)