FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193089
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGT2940.15226139241489675No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT2660.13776030742300183No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT2480.12843818135678367No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA2380.1232592224311069No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGACA252.3860401E-455.9999968
TCTGAGC503.4658078E-642.08
TCTTAGC350.001253175740.08
AGGCTAT350.001253175740.012
CGATCTG12350.036.2753035
TACACAG601.2113202E-535.0000045
AGTCCAA400.00241446735.022
CTGCGGT400.00241446735.014
CGATCTA4550.034.6153875
CTTCCGA26300.034.600761
TTCCGAT26650.034.4090082
CCGATCT26900.034.2193344
TCCGATC26950.034.155843
GATCTGA2900.033.7931066
TCTGGCC851.0572148E-732.9411778
TCTGGAC652.0950209E-532.3076938
ACATGCC552.9843024E-431.818188
AGCGTAA450.004299671431.11111340
CTTGACA450.004299671431.1111134
TCCGTTC450.004299671431.11111313
Produced by FastQC (version 0.11.2)