FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215637
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5420.25134833075956353No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3250.15071624999420322No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT3230.1497887653788543No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3190.14793379614815638No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3190.14793379614815638No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3140.14561508460978403No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2960.13726772307164353No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2930.13587649614862013No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2920.13541275384094567No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2730.1266016499951307No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2460.11408060768791997No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT2440.11315312307257103No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2360.10944318461117526No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2350.10897944230350079No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2330.10805195768815185No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2260.10480576153443055No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTT200.006944508852.49982569
GGTCCAA651.8371793E-1048.46137611
GTAGAAC350.001253521539.9998663
ATCTGTG701.9159415E-839.9998667
TTCCGAT16600.039.427582
TCCGATC16700.039.1914863
CTTCCGA16950.038.8289341
CCGATCT16600.038.584214
TAAGTGC400.002415130434.999886
GCGGACT400.002415130434.9998826
CACTTAT601.2120039E-534.999889
ATCTGAC1055.456968E-1033.333227
CGATCTG8600.032.9650085
TTTCGGA851.0560689E-732.9487081
CGATCTT4750.032.4209445
GTCTAGG652.0928734E-532.315081
ACTTATG652.0961976E-532.30758710
TTCCCGT652.0961976E-532.30758734
ATCTACT450.004300846331.1110087
GAATCTA450.004300846331.11100829
Produced by FastQC (version 0.11.2)