FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152878
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT2780.1818443464723505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCTCA150.002221205770.017
CGATTGT200.006940569352.524
TGCGGGG200.006940569352.58
TTCTCTA200.006940569352.57
TAGCCGG305.8633316E-446.6666645
ATCTAGG350.001252356940.07
TTAGTAT350.001252356940.026
CATGGAG400.002412899235.039
ATTAGTA400.002412899235.025
CTCGTAT806.1907485E-835.039
GTTTACT400.002412899235.029
TTAAGCA400.002412899235.04
AACTCCA400.002412899235.09
CTTTTTT851.0547592E-732.9411779
TCTTTTT851.0547592E-732.9411778
TAATAGA552.9814127E-431.8181844
AATAGAA552.9814127E-431.8181845
ACAGTCG450.004296896531.11111319
ATTACGA450.004296896531.11111331
TTCGTGA450.004296896531.11111310
Produced by FastQC (version 0.11.2)