FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144418
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2860.19803625586838203No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2700.1869573044911299No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2680.1855724355689734No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2200.15233558143721695No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1980.13710202329349527Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1880.13017767868271268Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCCT200.00694088152.497926
CCAAAAG200.00694088152.4979250
TTGAGTT400.002412926434.99861534
TCAATTC400.002412926434.99861525
CTGTTGC400.002412926434.9986159
TTTGAGT400.002412926434.99861533
CTTACAC400.002412926434.9986153
TCTAGAG450.00429694931.109882
GTCAATT450.00429694931.1098824
CGTCAAT450.00429694931.1098823
CTTTTTT1600.030.6237899
CCGTCAA500.007191223627.99889222
TGTTGCG500.007191223627.99889210
TCGTAGA500.007191223627.9988922
TACAGGA500.007191223627.9988922
TCTTTTT1800.027.2211468
GGGGTGT657.9112937E-426.93133716
CTAAGGT700.001221648724.999014
AGGGGGC851.3016419E-424.70490310
ATCTTTT2155.456968E-1222.7897957