Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s45.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 144418 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 56 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 286 | 0.19803625586838203 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 270 | 0.1869573044911299 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 268 | 0.1855724355689734 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 220 | 0.15233558143721695 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 198 | 0.13710202329349527 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 188 | 0.13017767868271268 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
AAACCCT | 20 | 0.006940881 | 52.49792 | 6 |
CCAAAAG | 20 | 0.006940881 | 52.49792 | 50 |
TTGAGTT | 40 | 0.0024129264 | 34.998615 | 34 |
TCAATTC | 40 | 0.0024129264 | 34.998615 | 25 |
CTGTTGC | 40 | 0.0024129264 | 34.998615 | 9 |
TTTGAGT | 40 | 0.0024129264 | 34.998615 | 33 |
CTTACAC | 40 | 0.0024129264 | 34.998615 | 3 |
TCTAGAG | 45 | 0.004296949 | 31.10988 | 2 |
GTCAATT | 45 | 0.004296949 | 31.10988 | 24 |
CGTCAAT | 45 | 0.004296949 | 31.10988 | 23 |
CTTTTTT | 160 | 0.0 | 30.623789 | 9 |
CCGTCAA | 50 | 0.0071912236 | 27.998892 | 22 |
TGTTGCG | 50 | 0.0071912236 | 27.998892 | 10 |
TCGTAGA | 50 | 0.0071912236 | 27.998892 | 2 |
TACAGGA | 50 | 0.0071912236 | 27.998892 | 2 |
TCTTTTT | 180 | 0.0 | 27.221146 | 8 |
GGGGTGT | 65 | 7.9112937E-4 | 26.931337 | 16 |
CTAAGGT | 70 | 0.0012216487 | 24.99901 | 4 |
AGGGGGC | 85 | 1.3016419E-4 | 24.704903 | 10 |
ATCTTTT | 215 | 5.456968E-12 | 22.789795 | 7 |