FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154788
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT1970.1272708478693439No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1560.10078300643460733No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1550.10013696152156497No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCC200.006940731352.570
CATAAGA200.006940731352.54
TTGTGTC350.001252404940.07
GGGTAAG400.002412991635.027
TCGGGTA400.002412991635.025
GTCGGGT400.002412991635.024
GTCCTAA450.00429706131.1111091
CTAAGGT450.00429706131.1111094
GCCATAA450.00429706131.1111091
TCCTAAG450.00429706131.1111092
TAGGGGT450.00429706131.1111094
ATCTTTT1401.2732926E-1130.07
TCAAGTG604.964622E-429.1666689
GGGTCAA500.007191405627.9999986
GTGGGTA500.007191405627.99999839
GTTCATG500.007191405627.9999989
TAAGGTA500.007191405627.9999985
AGGGACT500.007191405627.99999834
TAGCCTG657.9280825E-426.92307768
GCCCTAT657.9280825E-426.92307734
Produced by FastQC (version 0.11.2)