FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143483
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT2420.16866109573956495No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCATT200.00693970252.526
GTTAGTG200.00693970252.59
ATTGGAC200.00693970252.53
TAGGCTT200.00693970252.56
TAATGGA200.00693970252.534
TACCGAT200.00693970252.57
TCTAGGG404.5746263E-543.752
TCGTATG556.6583198E-638.1818240
CATTATC400.002412405335.03
TCAAGAG400.002412405335.046
TCTTCTG652.0912888E-532.30769350
CCCCCTA552.980504E-431.8181842
GACAGAT450.004296024331.1111097
AACTCAC450.004296024331.11110958
TTACCGG450.004296024331.11110931
TACCGGG450.004296024331.11110932
GATACCG604.962836E-429.1666665
CTCGTAT604.962836E-429.16666639
CGGGAAC604.962836E-429.16666615
TCTGCCG500.007189680427.9999988