FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193462
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT2090.10803155141578191No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGGGC200.006943445752.4998053
GAGTCTT305.867327E-446.66649225
GTGTCAC400.002411462435.008921
AGCTAGG400.002414523434.9998710
ATGTTCG400.002414523434.9998753
TAGACAG552.9843906E-431.8180645
TTAGACA450.00429977131.1109984
TATTACG450.00429977131.1109982
CAGCTAG450.00429977131.1109989
ATCCCCT853.9367696E-628.82342512
CTTCCAT500.00719591427.99989714
TAGGTGC500.00719591427.99989740
GCATTTA500.00719591427.99989735
GCCACTT500.00719591427.99989740
ACAGCTA657.9354725E-426.9229778
CTACAGA657.9354725E-426.9229774
ATCTGAG1101.0096337E-625.454457
GCACAGA700.00122289624.9999076
TGCAACG700.00122289624.99990712
ATCTGGG1851.4551915E-1124.5945037
Produced by FastQC (version 0.11.2)