FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258096
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6110.23673361849854319No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT4710.18249023619118468No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3770.1460696794991011No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA3690.14297005765296633No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA3610.13987043580683156No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3380.1309590229991941No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3260.12630959022999194No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA3260.12630959022999194No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3090.11972289380695554No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2980.11546091376852025No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2750.10654950096088277No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2730.10577459549934909No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2680.10383733184551486No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2640.10228752092244747No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGATC200.006933645352.52355624
ATTTACT200.006949610552.4930277
ATCTGTG855.638867E-1141.1709987
AATGTGC350.001253584540.0024346
TAAGGTG654.84828E-737.6946035
ATCTGAG852.566594E-937.0538987
CCGCAGT400.002410657235.015729
ATCTTAC400.00241754534.995357
TACAGCC400.00241754534.9953561
CTCGTAT1003.237801E-1034.99534639
CCGATCT22400.034.064574
TCCGATC22650.033.6885873
CTTCCGA22800.033.466951
TTCCGAT22950.033.248212
CGATCTG10350.033.1421175
ATATAAG552.986003E-431.8201222
ATCTTTT2450.031.4243957
ATGTATG802.453944E-630.63280314
CGTAAAT952.7992428E-729.48690823
CTTGCTT604.9662986E-429.17409912
Produced by FastQC (version 0.11.2)