Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s3.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 604605 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 2049 | 0.33889895055449426 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 1886 | 0.3119391999735364 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 1839 | 0.3041655295606222 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 1392 | 0.23023296201652318 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 956 | 0.15811976414353174 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 902 | 0.14918831303082178 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 842 | 0.13926447846114404 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATCGT | 35 | 0.0012554863 | 39.99877 | 9 |
TAGAATA | 50 | 1.7090038E-4 | 35.00182 | 4 |
CGTAATC | 80 | 6.240043E-8 | 35.00182 | 20 |
GCGGGTA | 60 | 1.2158502E-5 | 34.998924 | 14 |
TTTCGGA | 210 | 0.0 | 33.348858 | 1 |
ACGTAAT | 75 | 1.4896814E-6 | 32.66836 | 19 |
TAGCGCC | 45 | 0.00430751 | 31.110153 | 10 |
CTCGTAT | 125 | 1.05501385E-10 | 30.799053 | 39 |
TTAGAAT | 70 | 3.486898E-5 | 30.00156 | 3 |
AGCGTAA | 60 | 4.982427E-4 | 29.165771 | 50 |
GTATTAT | 50 | 0.007191342 | 28.013039 | 1 |
CTATAGG | 50 | 0.007191342 | 28.013039 | 1 |
AGCGTTT | 50 | 0.007208789 | 27.99914 | 16 |
TATGCAA | 50 | 0.007208789 | 27.99914 | 10 |
TGCTTGA | 140 | 3.947207E-10 | 27.499155 | 55 |
GTATGCC | 140 | 3.947207E-10 | 27.499155 | 42 |
GACGTAA | 90 | 6.1667233E-6 | 27.223637 | 18 |
GTCCTAT | 65 | 7.933336E-4 | 26.935616 | 1 |
CCTAGTC | 65 | 7.952531E-4 | 26.924479 | 3 |
TTCGGAA | 255 | 0.0 | 26.079786 | 2 |