FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences604605
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC20490.33889895055449426No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC18860.3119391999735364No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC18390.3041655295606222No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC13920.23023296201652318No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC9560.15811976414353174Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA9020.14918831303082178Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8420.13926447846114404No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATCGT350.001255486339.998779
TAGAATA501.7090038E-435.001824
CGTAATC806.240043E-835.0018220
GCGGGTA601.2158502E-534.99892414
TTTCGGA2100.033.3488581
ACGTAAT751.4896814E-632.6683619
TAGCGCC450.0043075131.11015310
CTCGTAT1251.05501385E-1030.79905339
TTAGAAT703.486898E-530.001563
AGCGTAA604.982427E-429.16577150
GTATTAT500.00719134228.0130391
CTATAGG500.00719134228.0130391
AGCGTTT500.00720878927.9991416
TATGCAA500.00720878927.9991410
TGCTTGA1403.947207E-1027.49915555
GTATGCC1403.947207E-1027.49915542
GACGTAA906.1667233E-627.22363718
GTCCTAT657.933336E-426.9356161
CCTAGTC657.952531E-426.9244793
TTCGGAA2550.026.0797862