FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67966
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT2740.40314274784451054No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT860.12653385516287557Illumina PCR Primer Index 11 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG680.10005002501250625No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTACT150.002215882370.030
ATCTTGG150.002215882370.012
CTCGTAT452.6533598E-854.44444739
TAAGTGG200.006924060652.50000417
ACCCTGC200.006924060652.5000041
TTAAGTG200.006924060652.50000416
TGCTCCA200.006924060652.50000421
TTTATGT200.006924060652.5000044
TACTAAC200.006924060652.5000047
TCTATGA200.006924060652.5000042
GGGGCTT200.006924060652.50000436
GTATGCC451.662318E-646.66666842
AAGGGGG451.662318E-646.66666869
TCTCGTA551.2883538E-744.54545238
AAAGGGG551.2883538E-744.54545268
CCGTCTT503.4326167E-642.047
AGGGGGT350.001247450640.05
ACCGTTA350.001247450640.08
TGCTTGA556.606697E-638.18181655
TATGCCG556.606697E-638.18181643
Produced by FastQC (version 0.11.2)