Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s37.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 198492 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 297 | 0.14962819660238197 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 246 | 0.12393446587267999 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 239 | 0.12040787538036797 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 215 | 0.1083167079781553 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 203 | 0.10227112427704894 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT | 199 | 0.1002559297100135 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GAGCTAG | 20 | 0.0069437055 | 52.499813 | 6 |
TATACCG | 20 | 0.0069437055 | 52.499813 | 5 |
TAGTGGT | 35 | 0.0012532823 | 39.999855 | 4 |
ACCCATG | 35 | 0.0012532823 | 39.999855 | 7 |
GTATGCC | 75 | 3.517016E-8 | 37.333202 | 42 |
CGGGTTA | 40 | 0.002414672 | 34.999874 | 15 |
TGTCTAA | 50 | 1.7054344E-4 | 34.999874 | 34 |
CTTGAAA | 80 | 6.2069375E-8 | 34.999874 | 57 |
AGACCAT | 90 | 1.7463572E-7 | 31.111002 | 6 |
ACACTTG | 45 | 0.004300035 | 31.111002 | 6 |
GTCTTCT | 95 | 2.804827E-7 | 29.47358 | 49 |
GCCGTCT | 95 | 2.804827E-7 | 29.47358 | 46 |
TAGAGTT | 75 | 5.565279E-5 | 27.9999 | 30 |
TCTGAGC | 50 | 0.0071963533 | 27.999899 | 8 |
GGTACAC | 50 | 0.0071963533 | 27.999899 | 3 |
ACACGCG | 50 | 0.0071963533 | 27.999899 | 52 |
CGTCTTC | 100 | 4.3937507E-7 | 27.999899 | 48 |
GCTTGAA | 100 | 4.3937507E-7 | 27.999899 | 56 |
CCGTCTT | 100 | 4.3937507E-7 | 27.999899 | 47 |
ATGCCGT | 100 | 4.3937507E-7 | 27.999899 | 44 |