FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198492
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2970.14962819660238197No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2460.12393446587267999No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2390.12040787538036797No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC2150.1083167079781553Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA2030.10227112427704894Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT1990.1002559297100135No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTAG200.006943705552.4998136
TATACCG200.006943705552.4998135
TAGTGGT350.001253282339.9998554
ACCCATG350.001253282339.9998557
GTATGCC753.517016E-837.33320242
CGGGTTA400.00241467234.99987415
TGTCTAA501.7054344E-434.99987434
CTTGAAA806.2069375E-834.99987457
AGACCAT901.7463572E-731.1110026
ACACTTG450.00430003531.1110026
GTCTTCT952.804827E-729.4735849
GCCGTCT952.804827E-729.4735846
TAGAGTT755.565279E-527.999930
TCTGAGC500.007196353327.9998998
GGTACAC500.007196353327.9998993
ACACGCG500.007196353327.99989952
CGTCTTC1004.3937507E-727.99989948
GCTTGAA1004.3937507E-727.99989956
CCGTCTT1004.3937507E-727.99989947
ATGCCGT1004.3937507E-727.99989944