FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203103
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2840.1398305293373313No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2580.1270291428487024No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2550.12555205979232212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCTA200.006943840552.547
TACTATA556.6720204E-638.1818162
TAGTTCT400.002414762535.04
TATACCT901.7466482E-731.1111135
GTTCTTA703.476859E-530.040
ACTATAC604.9700274E-429.1666683
AGAGGTT500.007196618228.00000265
AGAGCAG500.007196618228.0000025
CTATACC755.565776E-528.04
GTAAGCG906.14282E-627.22222320
GCGTATT657.936672E-426.92307724
TACCGGT657.936672E-426.92307730
CGTATTA700.001223080125.025
ACACCGG700.001223080125.06
TAGGACT700.001223080125.09
GTCGGTA700.001223080125.054
TAAGCGT851.3038424E-424.70588321
GCATAGG851.3038424E-424.7058831
AAAGTTA750.001827863623.33333459
TTCTTAT750.001827863623.33333441
Produced by FastQC (version 0.11.2)