FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163858
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2920.17820307827509185No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2110.12877003258919306No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1910.11656434229637858No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1840.11229235069389348No Hit
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT1840.11229235069389348No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1820.11107178166461205No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1810.11046149714997132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCAT200.006945527652.49221411
CTCGTAT503.4661152E-641.99377439
GCCTACA350.001247897640.030721
AGGGCCG350.00124977440.0184975
CCGTCTT556.670909E-638.1761647
ATATAAG400.002407934535.0161822
CCGAACT400.002407934535.0161824
TTACACT400.002407934535.0161824
AGTTAGA601.2089002E-535.00549361
GTATGCC601.2114328E-534.99481242
TTAGAGG652.0908472E-532.31276363
GTTAGAG652.0952193E-532.30290662
GCCGTCT652.0952193E-532.30290646
AGTAAAC652.0952193E-532.30290648
TACCCCA450.004288073631.1254965
ACGAATT450.004300910531.10649947
GTCCTAG802.4369474E-630.6485181
TGCTTGA802.4485926E-630.62980755
AAAGGGG703.46907E-530.00471168
GAATCAG952.8033173E-729.46931535