FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248883
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGT6810.27362254553344345No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT2990.12013677109324462Illumina PCR Primer Index 11 (95% over 22bp)
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2720.10928830012495831No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2590.10406496225133899No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAC150.002221408370.011463
CGATCTG10150.032.4190985
TCCATAC450.004298595231.1162013
ATATAGG450.004298595231.1162013
TGAACCG450.00430282631.1099510
CTTCCGA24400.030.2713471
TTCCGAT24550.030.086392
TGTACCG1052.0099833E-830.0049085
CCGATCT24650.029.8223254
TCCGATC25000.029.5448343
CTCGTAT1352.564775E-1028.51745439
TAATAGG500.00719393628.0045814
TAGGGAG500.00719393628.0045814
TATAATC500.00719393628.0045812
ATATTGG906.151906E-627.22120530
GTATTAG657.943458E-426.92207328
GTATGCC1508.749339E-1025.6657142
GATCTGG4550.025.388776
ATCTGCG1801.0913936E-1125.2768337
TCTGGGG1251.0912481E-725.2041243
Produced by FastQC (version 0.11.2)