FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108005
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT2950.2731355029859729No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2260.2092495717790843No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA1980.1833248460719411No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT1770.16388130179158372No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1760.16295541873061434No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG1710.15832600342576733No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC1710.15832600342576733No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC1620.14999305587704273No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG1600.14814128975510393No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1520.14073422526734874No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC1380.12777186241377714No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT1360.12592009629183834No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA1320.12221656404796073No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC1280.11851303180408315No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1280.11851303180408315No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1170.10832831813341974No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCA150.00221943570.000014
TAATAGT200.006935080552.528
AAGTAAT200.006935080552.525
TAAAGGC200.006935080552.54
TTTGCAC200.006935080552.520
ACTCCGT200.006935080552.535
ATAAGAA506.1316314E-848.9999964
ATATAAG451.6715403E-646.6666682
TATAAGA451.6715403E-646.6666683
TAAGAAC451.6715403E-646.6666685
AAGAACT503.4515815E-642.06
TAATACG601.2063894E-535.00000420
AATACGC601.2063894E-535.00000421
GGGCGAT501.7002625E-435.011
AAACAGT400.002409773635.044
AGGTCTT501.7002625E-435.07
TAATCTC400.002409773635.069
GGTAATA652.0865291E-532.30769318
CATATAA652.0865291E-532.3076931
GAACTCC652.0865291E-532.3076938