FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199689
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4360.2183395179504129No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2690.134709473230874No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2630.13170480096550136No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2560.12819934998923324No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG2560.12819934998923324No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2560.12819934998923324No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2540.12719779256744237No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2530.12669701385654694No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2480.12419312030206972No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2400.12018689061490619No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT2270.11367676737326542No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2140.10716664413162467No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC2110.10566430799893835No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2100.10516352928804291No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGTA252.386322E-455.99964
ATTGCTT200.006943865752.4996228
ACTTATG352.0813122E-549.9996410
GGTCTAA350.001250237540.019761
ACTATAC459.174995E-538.8886073
CGTATTA459.174995E-538.88860725
TACCGGT459.174995E-538.88860730
CGGGTTA501.7055067E-434.99975215
CTGGTAA501.7055067E-434.99975224
GTATGTA501.7055067E-434.99975216
GTTCCGA501.7055067E-434.99975233
TACTATA501.7055067E-434.9997522
TTATGTT400.002414749534.9997512
CTTGGTC400.002414749534.9997538
TGGGACG400.002414749534.9997510
TATGTAT652.09548E-532.3074613
GTCCTAA552.9760264E-431.8338991
CTCGTAT552.9847905E-431.81795539
GGGTAAG552.9847905E-431.81795527
TAAGTTC552.9847905E-431.81795530