FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s27.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s27.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176779
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT4220.23871613709773218No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG3080.17422883939834483No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA2990.16913773694839318No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT2700.15273307349854903No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA2700.15273307349854903No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT2440.13802544419868876No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2410.13632841004870488No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG2390.13519705394871562No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2220.125580527098807No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2140.12105510269884998No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA2080.11766103439888223No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2020.11426696609891447No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA1970.11143857584894133No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA1880.10634747339898971No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT1870.10578179534899508No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT1840.1040847611990112No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC1820.10295340509902193No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG1800.1018220489990327No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAAT200.006942357452.523
CATGTAT751.4551915E-1146.66666825
GCATGTA855.638867E-1141.1764724
TGTATTA801.4115358E-939.37527
ATAAGGT1007.2759576E-1238.5000044
AGCTCTA1007.2759576E-1238.50000433
GTATTAG852.5538611E-937.05882328
GGTCCAA852.5538611E-937.05882311
TGTTTTA951.8553692E-1036.84210614
TTAATTG951.8553692E-1036.84210618
CCATAAG951.8553692E-1036.8421062
CATGTTT951.8553692E-1036.84210612
ATTGCAT1051.2732926E-1136.66666821
TTCCGGG501.7046448E-435.0000042
GTTAACG1003.2196112E-1035.00000466
ATCTTAG501.7046448E-435.0000047
GGTTCAT1003.2196112E-1035.0000048
GTGCAAG400.002413917635.011
CCGGTAA708.644656E-735.016
TGGCTCG400.002413917635.05