FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249831
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT5880.23535910275346134No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5220.20894124428113406No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA4880.19533204446205635No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA4150.1661122919093307No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC3960.15850715083396374No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT3820.15290336267316706No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3580.14329686868322986No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC3410.13649226877369103No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3360.13449091585912076No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT3300.13208929236163647No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2940.11767955137673067No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2890.11567819846216043No Hit
CATCTAAGGAAGGCAGCAGGCGCGTAAATTACCCACTCCCAGCTCGGGGA2770.11087495146719181No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC2710.10847332796970752No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2700.10807305738679347No Hit
CCTTATGGGACATGTGCTTTTATTAGGCTAAAACCAAGCGATCGCAAGAT2690.10767278680387943No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2660.10647197505513728No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT2630.10527116330639513No Hit
ATACAGGACTCATATCCGAGGCCCTGTAATTGGAATGAGTACACTTTAAA2620.10487089272348109No Hit
CTTTAAATCCTTTAACAAGGACCAATTGGAGGGCAAGTCTGGTGCCAGCA2550.10206899864308272No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA2530.10126845747725462No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGT252.3853118E-456.009931
GTAGGAT200.006946330452.49883
TATAACA200.006946330452.49882
TGTAACC200.006946330452.49884
GTACAGG953.6379788E-1240.53351
TACTATA1151.8189894E-1236.5209052
CCTTTAT1102.3646862E-1134.9992039
GGGAACG501.7068902E-434.999216
GATAGGT400.002416093834.99927
ACTATAC1154.0017767E-1133.4774933
ATACCTT1154.0017767E-1133.4774936
CTCGCGT1700.032.94701829
GGATTAT652.0978032E-532.3069536
GCGTATT1206.548362E-1132.082624
CCTATCT1750.031.99926870
TTTCGGA450.004298338731.1166271
ATAGCCG450.00430255331.110453
TTCTTAT1357.2759576E-1231.110441
ATATAAG901.7497405E-731.11042
TAGGCTG450.00430255331.11045
Produced by FastQC (version 0.11.2)