H3W35AFXY_n01_adr3wtzt14s25.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s25.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1745
Sequences flagged as poor quality	0
Sequence length	76
%GC	56

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT	590	33.810888252149	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	132	7.564469914040115	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT	35	2.005730659025788	Illumina PCR Primer Index 10 (95% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	33	1.8911174785100286	No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCT	15	0.8595988538681949	Illumina PCR Primer Index 10 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	15	0.8595988538681949	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	10	0.5730659025787965	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATATCGTATGCCGT	10	0.5730659025787965	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	4	0.2292263610315186	No Hit
ATCTAGAGACGTGTGCTCTTCCGATCTAGAGACGTGTGCTCTTCCGATCT	3	0.17191977077363896	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCACATCCCGGCCAGGGGCGGATCACTTTCCGGGACTGCTCCACCATCG	2	0.1146131805157593	No Hit
GCTTCAGGATGCTCTTGGCCGGCACCTCGGGCGGCGGCGCACGGTCGCTG	2	0.1146131805157593	No Hit
GTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGC	2	0.1146131805157593	No Hit
GTGGTGCAGCCTTACTGGTTCGGTGATGCTGAGTTCAAGGCCTGTCTCTT	2	0.1146131805157593	No Hit
GTTATCTAGAGTTCAACCAATATAACGATCTTGCGATCGCTTGGTTTTAG	2	0.1146131805157593	No Hit
GTATACAGTGTCTGTGGGGGGAGTCCAAGCTGCTGAATATCTGTCTCTTA	2	0.1146131805157593	No Hit
CACGCACACCGTCAATCAGAACCAGCGTCTGATTGGAACCGGCGCCGCGC	2	0.1146131805157593	No Hit
TACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	2	0.1146131805157593	Illumina PCR Primer Index 10 (95% over 23bp)
GCATCCTGCTGGTGTTCAACATCTTCGGGCCGTTCATGGTGCAGACCCTG	2	0.1146131805157593	No Hit
ATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAACAT	2	0.1146131805157593	No Hit
CGCCTCGACCGTGCGGGCGTAGCGCGGCGCTGAGGCGAGCGTCTCGCCGG	2	0.1146131805157593	No Hit
ACCGCGTAACGCGCAGGCACGCTTTCAGCGCGGCGTGGCACTTGCCGGCC	2	0.1146131805157593	No Hit
CAAGGGAAGTGCCCCCCGGCCAAACCCGGGGGGCACTTCCCTTGGGGTCA	2	0.1146131805157593	No Hit
CTTCTGCCTGAAAAGGTGAACGTTGCTGAGCACATCGACGCAGGCGGCTT	2	0.1146131805157593	No Hit
AACGCAACAGGCGCGAGGATGGCAAGCGCGATGACGCCAAGAATGGTGTA	2	0.1146131805157593	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTCTATCTCGTATGCCGT	2	0.1146131805157593	No Hit
CCATCAACCGCCCGGAGGCAAGCGGGTTCGGCCGTTGGCTGGATCTGCTC	2	0.1146131805157593	No Hit
GAACTATCGTGTGCGGTCAAAGTCGTCCTTCACGAGATGTTTGCCTCCAG	2	0.1146131805157593	No Hit
GACGGGCGGTTGGGTGGATGCCGCAAAGCGCGAAACGATGATTGCCGTCG	2	0.1146131805157593	No Hit
TCTCTAGATCGGAAGAGCACACGTCTCTAGATCGGAAGAGCACACGTCTC	2	0.1146131805157593	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTAAGGTACTTAGCTCATCACAAATGAGGTTAAGAAACACATTTTCATAT	2	0.1146131805157593	No Hit
AAGATACGATTTATTATGAACCAAAACAAACAAGTAAATTCGAACAAACA	2	0.1146131805157593	No Hit
ACGGTTGACTCCGAATTCGGCCGCACGACCGACCCGGTGCGCATGTACAT	2	0.1146131805157593	No Hit
ACCGTCTGTGGCCCTGACTAGGCGGCGAAGATCGCTGATCTCACGTACCG	2	0.1146131805157593	No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA	2	0.1146131805157593	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGGGCCTATCTCGTATGCCGT	2	0.1146131805157593	No Hit
AGCTACGACCGCAATCGCTCTCGCCTTCAGCGCGGCACCTGCATCGGCGC	2	0.1146131805157593	No Hit
GGGCTGGGCAACAGTTGGGCGCCAGCTCCGGTACAGATTCGACGGAGAAT	2	0.1146131805157593	No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	2	0.1146131805157593	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACTAAGGTCAGTAGCATGCTGTATTTTCTCTTCTGAATTCTTTCTACATT	2	0.1146131805157593	No Hit
CAAGAAGACCGGCGGCTTCGACCTCGAAGACTTCAAGGCCCAGATCGGCC	2	0.1146131805157593	No Hit
GGCATCGCCCACCTGCTCGCCGATCTTCGTGGCCGAGATGCCCATGTGCG	2	0.1146131805157593	No Hit
GGTCAAGCGCGTTGCTGAGCCACAGTGCGGCCGTCACCGAGCCGGTCAGC	2	0.1146131805157593	No Hit
CGTGCCGGCCATCGCTGGCAGGCTTGCCATCAGCGCCAGCGCCAGCGCCA	2	0.1146131805157593	No Hit
CGACAAGATCGTCGAAACGCTGCTCCGGCTGCAAGACGATGAAACTCTGC	2	0.1146131805157593	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	60	1.6152626E-9	46.666664	1
TTATACA	70	9.458745E-11	45.0	2
TATACAC	75	1.8553692E-10	42.0	3
AGCCTAT	85	6.348273E-10	37.058823	32
AGGGGGG	85	6.348273E-10	37.058823	70
GTATGCC	85	6.348273E-10	37.058823	42
TGCCGTC	85	6.348273E-10	37.058823	45
TATGCCG	85	6.348273E-10	37.058823	43
GAAAAAA	85	6.348273E-10	37.058823	60
GAGCCTA	85	6.348273E-10	37.058823	31
TTCTGCT	85	6.348273E-10	37.058823	52
CCGTCTT	85	6.348273E-10	37.058823	47
AAGGGGG	85	6.348273E-10	37.058823	69
TCTGCTT	85	6.348273E-10	37.058823	53
TGCTTGA	85	6.348273E-10	37.058823	55
ATGCCGT	85	6.348273E-10	37.058823	44
AAAGGGG	85	6.348273E-10	37.058823	68
GTCTTCT	85	6.348273E-10	37.058823	49
CGTATGC	85	6.348273E-10	37.058823	41
GCTTGAA	85	6.348273E-10	37.058823	56

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content