FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290671
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA21000.7224662935070922No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG19690.6773981580549832No Hit
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT19440.6687973688465654No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG18700.6433390327896488No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC15310.5267123311235039No Hit
GACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCAC13970.4806121009663847No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG13700.4713232486212935No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC12150.41799835552910336No Hit
GTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGA11660.40114080868060453No Hit
GATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGA10850.37327425164533096No Hit
GCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATAC10410.3581368626385157No Hit
GGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTG10140.34884801029342455No Hit
TCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGG10110.3478159155884144No Hit
CTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAA9500.32682998991987505No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCG9290.31960532698480415No Hit
TAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAA9270.3189172638481307No Hit
GCAATAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCT9000.30962841150303955No Hit
CATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGG9000.30962841150303955No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA8980.3089403483663661No Hit
GTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGC8960.30825228522969267No Hit
CCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGT8690.2989634328846015No Hit
GGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATG8620.2965552119062445No Hit
CTACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAA8580.2951790856328977No Hit
ACAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAAC8470.29139473838119384No Hit
CTTCCGATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTT8380.28829845426616346No Hit
GGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAA8050.27694541251105204No Hit
ATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTCGGTCTAG7800.26834462330263426No Hit
GTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACC7800.26834462330263426No Hit
CCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTG7510.25836770782086965No Hit
GGGATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT7320.25183110802247216No Hit
TAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATT6980.240134034699024No Hit
TCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACCTG6880.2366937190156569No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG6850.23566162431064674No Hit
CAATGAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACC6710.23084518235393278No Hit
AGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACA6560.22568470882888214No Hit
CTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGAC6490.22327648785052515No Hit
CTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATGAACTTGGAATT6400.22018020373549477No Hit
AGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGC6310.21708391962046436No Hit
GGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATT6100.20985925668539346No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC5830.20057040434030227No Hit
TAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATG5560.19128155199521107No Hit
GTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCAT5520.18990542572186422No Hit
GCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGT5280.18164866808178318No Hit
GTTCAGCGGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACT5100.1754560998517224No Hit
CGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCA5100.1754560998517224No Hit
GTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACAATGAAAGTATC4930.1696075631899983No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4920.1692635316216616No Hit
GACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAACAGGTCTGTGAT4830.1661672475066312No Hit
AGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTC4780.16444708966494764No Hit
GTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCA4680.16100677398158056No Hit
AGCTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATA4670.16066274241324383No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGA4560.15687839516154004No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT4540.1561903320248666No Hit
TCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCC4470.15378211104650963No Hit
GTACAAAGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACT4470.15378211104650963No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4460.15343807947817292No Hit
TTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCACAGACC4340.1493097006581324No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT4330.14896566908979567No Hit
ATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCACGCGCGCTACA4250.146213416543102No Hit
AGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGCTGCAC4190.14414922713308173No Hit
GAAAGTATCAACGTGTATTTCCTAGACCGAGAGGTCCGGGTAAACCGCTG4170.14346116399640832No Hit
CTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAAT4130.14208503772306147No Hit
CTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAA4120.14174100615472476No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG4010.13795665890302095No Hit
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTATTGCTAGACG3970.1365805326296741No Hit
GGTTTACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTG3900.13417231165131713No Hit
GAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATC3880.13348424851464372No Hit
TCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGG3810.13107602753628672No Hit
ATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAGCTAGTGTCCTT3750.12901183812626646No Hit
ACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTG3660.12591555401123608No Hit
GGGTAAACCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGT3620.12453942773788923No Hit
GAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTAC3590.1235073330328791No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA3490.120067017349512No Hit
CTTCTTAAATGGACAAATTGCGTCTAGCAATAATGAGATTGAGCAATAAC3400.11697073323448161No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA3360.11559460696113474No Hit
CCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGC3330.11456251225612463No Hit
GTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCGGACCTCTC3280.11284235441444107No Hit
GATTAATAGAAGCAGTTTGGGGGCATTAGTATTACGACGCGAGAGGTGAA3250.11181025970943093No Hit
GTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAG3200.11009010186774738No Hit
ACCCGGACCTCTCGGTCTAGGAAATACACGTTGATACTTTCATTGTAGCG3160.10871397559440055No Hit
GGACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGT3120.1073378493210537No Hit
GTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGATTAATGAAAAC3090.10630575461604358No Hit
CAGTTTCAGTTCACAATCCCAAGCATGAAAGTGGTTCAGCGGTTTACCCG3090.10630575461604358No Hit
GATCTGGGCAGGGACGTAATCAATGCGAGTTAATGACTCACACTTACTGG3080.10596172304770685No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA3050.10492962834269673No Hit
GATACTTTCATTGTAGCGCGCGTGCAGCCCAGGACATCTAAGGGCATCAC3040.10458559677436001No Hit
CCGCTGAACCACTTTCATGCTTGGGATTGTGAACTGAAACTGTTCACATG3010.10355350206934988No Hit
ATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACA3000.10320947050101317No Hit
GAGTTAATGACTCACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTT2960.10183334422766634No Hit
GGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTAGACCG2950.10148931265932962No Hit
AACTGAAACTGTTCACATGAACTTGGAATTCCCAGTAAGTGTGAGTCATT2920.10045721795431947No Hit
CACACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATC2920.10045721795431947No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTAG150.002223400969.9986310
CAGCGTA200.006947379652.4989669
GGGTCCG200.006947379652.49896640
TTAGTCA200.006947379652.49896642
CTGGCTA200.006947379652.4989669
CTGTGCG200.006947379652.4989669
GCTTAGG352.081714E-550.0076181
TGTACTG506.181108E-848.999045
CGATCTG11250.045.421335
TCCGATC21650.040.899893
TCCTCTG602.5912777E-740.8325346
TTCCGAT21950.040.500342
CCGATCT22100.040.067084
CTTCCGA22200.040.0511471
ATTAGGG350.00125434339.9992142
CTGTACT701.9201252E-839.9992144
TCTAGGC350.00125434339.9992148
TCTGGCT350.00125434339.9992148
ATCTGGG5250.039.999217
TCTGGGC3900.039.48648
Produced by FastQC (version 0.11.2)