FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93734
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC2900.30938613523374653No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2670.2848486141634839No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC2350.25070945441355325No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC1830.19523331981991593No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA1550.16536155503872663Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC1500.16002731132779996Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT150.002218515470.013
ATGAAAC150.002218515470.068
TAAATGT150.002218515470.026
AGTAGGT150.002218515470.059
CTAGAAC200.00693222552.53
AGAGTGT200.00693222552.56
GTTACCT200.00693222552.540
ACCTATC200.00693222552.515
TTAAAGC200.00693222552.53
GCGGACA200.00693222552.555
AGCTCCC200.00693222552.535
AGGCTTC200.00693222552.510
GACCCGG400.002408148535.011
CACAAGT400.002408148535.070
GTAATCT1001.2343662E-831.50000270
AGCACAA450.00428848831.11111368
GAATCAG450.00428848831.11111335
ATAACAT450.00428848831.11111348
CCCAGAA703.4570978E-530.02
TCTGACC604.9498514E-429.1666668
Produced by FastQC (version 0.11.2)