FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224499
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT3070.13674893874805677No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC2930.13051283079211934No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC2890.12873108566185149No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA2790.1242767228361819No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT2570.11447712461970878No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG2500.11135907064174005No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC2260.100668599860133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCAT200.00694539852.49883331
TATCAAG350.001252375340.0080222
GTAATAT350.001253748539.99910770
GAAGATA400.00241292635.007026
TGTTAGA400.00241292635.007022
CTAGTGT400.002415565334.9992240
GCTAGTG400.002415565334.9992239
AAGATAT400.002415565334.999227
GTGATTA708.6600267E-734.9992189
ATATGTG751.4824363E-632.6732185
TCTAGGC652.096851E-532.3069738
CCGTCTT652.096851E-532.30697347
TATATAA552.9823283E-431.8245645
TAGGCCG552.9862305E-431.81747210
TATATGT802.4531E-630.6311424
GTACAAC802.4573692E-630.6243258
GCCGTCT703.4790006E-529.9993346
TGCCGTC703.4790006E-529.9993345
TAGCGTA604.9723237E-429.1660187
CTTATAT853.9342085E-628.829312