FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335990
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC16060.4779904163814399No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC14990.4461442304830501No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC14320.4262031608083574No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC11450.34078395190333044No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT6850.2038751153308134No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC6390.19018423167356172Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5960.1773862317330873No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5850.17411232477157057Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5080.1511949760409536No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5060.15059972022976875No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4680.13928985981725647No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT4560.13571832495014732No Hit
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC4330.12887288312152148No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT4090.1217298133873032No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4040.12024167385934106No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG3870.11518199946426977No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG3690.10982469716360607No Hit
GTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAGTTCAA3680.10952706925801362No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT3370.10030060418464837No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTAT207.921047E-569.9989622
GATAATA200.006948184752.4992182
TAACCGA350.001254592739.99940544
CGACTTA350.001254592739.99940521
ACCGAAT400.002417181234.9994846
GCTCTAA400.002417181234.999481
CGAATAG400.002417181234.9994848
TGCAACG2750.034.3682419
GCAACGG2800.033.7545220
AACGGGT2800.033.74949622
CGGGTAA2900.032.5857224
AACGGCT2600.032.30721370
GGGTAAC2950.032.0334225
AGGACTA2950.032.033425
AAACGGC2650.031.69764169
TAACGGG2800.031.24953528
TACCATG3250.031.23030310
TAGGACT3150.031.110654
GAGTATC450.004304477531.1106477
ATGGTTG3050.030.98775514
Produced by FastQC (version 0.11.2)