FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239550
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT4020.16781465247338762No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAATA150.00222311769.997082
GTAATAT200.00694650152.4978170
CTAGCAC350.001254037439.998333
TCTAGGG556.678949E-638.1802252
ATCTGTG904.480171E-934.9985437
CTATGCA501.7068707E-434.998549
TAGAATA501.7068707E-434.998544
TAGCACT400.002416119434.998544
TTCCTAT400.002416119434.998542
TTAGAAT751.4857997E-632.6653023
CCGATCT20850.031.5574344
TTCCGAT21200.031.5317042
CTTCCGA21450.031.1642021
TCCGATC21450.031.1642023
TTGTACC450.004302600431.1098164
ACGATTC450.004302600431.10981612
GCTTAAG450.004302600431.10981670
TACTAAT450.004302600431.10981641
GTACTGG802.4586552E-630.6237221
ATTAATG604.973877E-429.1654539
Produced by FastQC (version 0.11.2)