FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96906
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT1430.14756568220750005No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTGCACGAAT1170.12073555816977277No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTACAA200.00692080852.5232328
ATCTATA200.006934958552.4961282
CATTGCG200.006934958552.49612818
GTTAGTG200.006934958552.49612851
GAGTTAG200.006934958552.49612849
ACGATGG551.2967757E-744.5421757
GTATGCC503.449637E-641.99690642
GCACGAA1050.039.9970543
TCTAGGG350.001250540839.997052
CGAATGG1050.039.9970546
ACGAATG1050.039.9970545
TGGCGTA801.3969839E-939.37209750
GGCGTAA901.0186341E-1038.8860251
TGTCTCC901.0186341E-1038.8860270
CACGAAT1007.2759576E-1238.4971644
GTTTACC556.615808E-638.19871529
AAAAGGG556.6393204E-638.17900567
AATGGCG852.5302143E-937.0560948
GAATGGC951.8371793E-1036.8393947
GCGTAAC951.8371793E-1036.8393952