FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361913
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC11050.30532199727558834No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC10600.29288806978472726No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC9580.26470450080544217No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8530.23569200332676635No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5310.14672034439216056Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA5100.1409178448964254Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTATT200.006944737452.5066342
TCTATAT501.7069047E-435.004422
TAACATG450.004301937831.1150444
TAGGATC450.004301937831.1150444
CTGAGGT703.4813027E-530.0037924
GGTTATA500.00720436227.99966811
ATAATAG657.9428783E-426.926483
TCTTCTG1305.3842086E-926.92275850
GCCGTCT1056.7558176E-726.66635146
ATGTTAT808.647138E-526.2533171
GTATGCC1101.0137883E-625.45424542
TCTTGAG700.00122402925.0031592
TGCTTGA1151.4932521E-624.34753855
TCTTTTT4050.023.33305713
ACGAGTG750.001830742123.33305769
TGAGCGC750.001830742123.33305719
CTTTTTT4200.022.49973314
GTACCGA800.002661698321.8777646
GTCCTAA1454.5850538E-721.7268831
GTGTTGG1003.9231032E-421.0026511
Produced by FastQC (version 0.11.2)