H3W35AFXY_n01_adr3wtzt14s18.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H3W35AFXY_n01_adr3wtzt14s18.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	330827
Sequences flagged as poor quality	0
Sequence length	76
%GC	54

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC	620	0.18740912924277645	No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC	574	0.17350458094411883	No Hit
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAT	526	0.15899548706725872	No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC	516	0.15597275917624617	No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC	467	0.14116139251028484	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA	413	0.1248386618988172	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC	409	0.1236295707424122	Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTCACG	15	0.002223538	69.9997	38
GTCCATA	20	0.0069394973	52.515648	1
GATATAA	50	3.4662353E-6	42.01252	1
GGGTATT	85	2.568413E-9	37.058662	16
CTAATTC	40	0.0024170026	34.999847	3
GATATGG	55	2.9836583E-4	31.827667	1
CTGTATA	45	0.0043041604	31.110975	28
GGGCTTA	45	0.0043041604	31.110975	29
ATAAATC	45	0.0043041604	31.110975	46
ATTCTGT	45	0.0043041604	31.110975	56
TAGAGTC	60	4.976838E-4	29.166542	37
GGTATGG	50	0.007192589	28.008345	1
CGGGTAT	75	5.5763863E-5	27.99988	15
CCTAGAA	50	0.0072032153	27.99988	21
CATACAT	50	0.0072032153	27.99988	3
GGTTATA	50	0.0072032153	27.99988	2
TCTGAGT	65	7.9474924E-4	26.922962	8
GGTCTAA	65	7.9474924E-4	26.922962	11
TGTACTG	65	7.9474924E-4	26.922962	5
TCTAGGC	80	8.652619E-5	26.249887	8