FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25043
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT5282.1083735974124505No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT830.331429940502336TruSeq Adapter, Index 12 (95% over 22bp)
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT490.19566345885077666No Hit
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA460.1836840634109332No Hit
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG400.15972527253124627No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG360.14375274527812162No Hit
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC340.13576648165155933No Hit
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG330.13177334983827815No Hit
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT320.127780218024997No Hit
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT320.127780218024997No Hit
ATTTAATATATTTGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCA310.12378708621171586No Hit
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG300.1197939543984347No Hit
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC300.1197939543984347No Hit
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG290.11580082258515352No Hit
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA280.11180769077187237No Hit
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG280.11180769077187237No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA280.11180769077187237No Hit
GTGTAAGACAGATTGATAGCTCTTTCTCGAATCTATGGGTGGTGGTGCAT260.10382142714531006No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTGGG150.00219946470.012
CCGATCT650.059.2307664
CTTCCGA650.059.2307661
CGATCTG553.274181E-1157.2727245
GATCTGC252.345925E-456.0000046
TCCGATC700.055.0000043
TATGCCG452.5633199E-854.44444743
TGCTTGA452.5633199E-854.44444755
ATGCCGT452.5633199E-854.44444744
GCCGTCT452.5633199E-854.44444746
CTGGGCA200.00687315152.59
TCTGGGC200.00687315152.58
TAAAACG200.00687315152.517
GCTGTGG200.00687315152.511
TGGGCAG200.00687315152.510
TGAAAAA552.1354936E-950.9090959
GATCTGG352.0289035E-550.0000046
TGCCGTC505.8807927E-849.00000445
CCGTCTT505.8807927E-849.00000447
TCTTCTG505.8807927E-849.00000450