Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s17.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25043 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 46 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT | 528 | 2.1083735974124505 | No Hit |
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT | 83 | 0.331429940502336 | TruSeq Adapter, Index 12 (95% over 22bp) |
GACGTAATCAATGCGAGTTAATGACTCACACTTACTGGGAATTCCAAGTT | 49 | 0.19566345885077666 | No Hit |
GTCCTGGGCTGCACGCGCGCTACAATGAAAGTATCAACGTGTATTTCCTA | 46 | 0.1836840634109332 | No Hit |
GTCTAGGAAATACACGTTGATACTTTCATTGTAGCGCGCGTGCAGCCCAG | 40 | 0.15972527253124627 | No Hit |
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG | 36 | 0.14375274527812162 | No Hit |
CACTTACTGGGAATTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCC | 34 | 0.13576648165155933 | No Hit |
CTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAG | 33 | 0.13177334983827815 | No Hit |
ATTATAAGGACACTAGCTTCTTAAATGGACAAATTGCGTCTAGCAATAAT | 32 | 0.127780218024997 | No Hit |
ATATTAAATAGATATCTTCAGGATTATGGTGCTGAAGCTTATGTAGCCTT | 32 | 0.127780218024997 | No Hit |
ATTTAATATATTTGAGTCTCGTTCGTTATCGGAATTAACCAGACAAATCA | 31 | 0.12378708621171586 | No Hit |
GTTATTGCTCAATCTCATTATTGCTAGACGCAATTTGTCCATTTAAGAAG | 30 | 0.1197939543984347 | No Hit |
TAATGAGATTGAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCTGGGC | 30 | 0.1197939543984347 | No Hit |
TTCCAAGTTCATGTGAACAGTTTCAGTTCACAATCCCAAGCATGAAAGTG | 29 | 0.11580082258515352 | No Hit |
GGATTATGGTGCTGAAGCTTATGTAGCCTTCATTCATGTTGGCAGTAAAA | 28 | 0.11180769077187237 | No Hit |
TTCCTAGACCGAGAGGTCCGGGTAAACCGCTGAACCACTTTCATGCTTGG | 28 | 0.11180769077187237 | No Hit |
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA | 28 | 0.11180769077187237 | No Hit |
GTGTAAGACAGATTGATAGCTCTTTCTCGAATCTATGGGTGGTGGTGCAT | 26 | 0.10382142714531006 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTGTGGG | 15 | 0.002199464 | 70.0 | 12 |
CCGATCT | 65 | 0.0 | 59.230766 | 4 |
CTTCCGA | 65 | 0.0 | 59.230766 | 1 |
CGATCTG | 55 | 3.274181E-11 | 57.272724 | 5 |
GATCTGC | 25 | 2.345925E-4 | 56.000004 | 6 |
TCCGATC | 70 | 0.0 | 55.000004 | 3 |
TATGCCG | 45 | 2.5633199E-8 | 54.444447 | 43 |
TGCTTGA | 45 | 2.5633199E-8 | 54.444447 | 55 |
ATGCCGT | 45 | 2.5633199E-8 | 54.444447 | 44 |
GCCGTCT | 45 | 2.5633199E-8 | 54.444447 | 46 |
CTGGGCA | 20 | 0.006873151 | 52.5 | 9 |
TCTGGGC | 20 | 0.006873151 | 52.5 | 8 |
TAAAACG | 20 | 0.006873151 | 52.5 | 17 |
GCTGTGG | 20 | 0.006873151 | 52.5 | 11 |
TGGGCAG | 20 | 0.006873151 | 52.5 | 10 |
TGAAAAA | 55 | 2.1354936E-9 | 50.90909 | 59 |
GATCTGG | 35 | 2.0289035E-5 | 50.000004 | 6 |
TGCCGTC | 50 | 5.8807927E-8 | 49.000004 | 45 |
CCGTCTT | 50 | 5.8807927E-8 | 49.000004 | 47 |
TCTTCTG | 50 | 5.8807927E-8 | 49.000004 | 50 |