FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53696
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT2450.4562723480333731No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.16761025029797377No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG850.15829856972586412No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT830.15457389749702027No Hit
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT660.12291418355184743TruSeq Adapter, Index 11 (95% over 23bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT620.11546483909415971No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA600.11174016686531585No Hit
CATATAAGAACTCCACCGGTAATACGCTTACATACATAAAGGTATAGTAC580.10801549463647199No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG560.10429082240762813No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT550.10242848629320621No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA540.10056615017878427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGG308.272169E-658.3333323
CGTCTTC252.3704788E-456.00000448
GTCTTCT252.3704788E-456.00000449
GCCGTCT252.3704788E-456.00000446
CGGCGTG200.006916148652.564
GCTATCG200.006916148652.531
GGTCCAA200.006916148652.511
AACGAAA200.006916148652.555
CTGCTTG305.829155E-446.66666454
TGCTTGA305.829155E-446.66666455
TATGCCG305.829155E-446.66666443
GTTAACG305.829155E-446.66666466
GTATGCC305.829155E-446.66666442
TGTTAAC305.829155E-446.66666465
CAACTGG305.829155E-446.66666444
ATGCCGT305.829155E-446.66666444
TGCCGTC305.829155E-446.66666445
TCTTCTG404.5362136E-543.7550
GTAACTG404.5362136E-543.7560
CTCGTAT350.001245100540.00000439
Produced by FastQC (version 0.11.2)