Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s15.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 308742 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 877 | 0.284055943149944 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 806 | 0.2610593958709861 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 748 | 0.24227348400930226 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 539 | 0.17457942230082074 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 483 | 0.15644130050333288 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT | 469 | 0.15190677005396092 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 434 | 0.140570443930531 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GTATAAA | 15 | 0.0022233592 | 70.0 | 1 |
TATATAC | 20 | 0.0069472454 | 52.5 | 3 |
GTCTAAT | 20 | 0.0069472454 | 52.5 | 1 |
CGTAATT | 20 | 0.0069472454 | 52.5 | 12 |
CCATACA | 40 | 0.0024167022 | 35.0 | 1 |
GTATAGT | 60 | 1.2136716E-5 | 35.0 | 1 |
CGAGTGC | 65 | 2.0990688E-5 | 32.307693 | 70 |
TATGTGA | 55 | 2.988422E-4 | 31.81818 | 6 |
AATACGC | 45 | 0.0043036286 | 31.111109 | 21 |
TTAGACA | 70 | 3.4826637E-5 | 30.0 | 4 |
TATATGT | 60 | 4.9759523E-4 | 29.166666 | 4 |
GTCCTAA | 75 | 5.5750326E-5 | 28.0 | 1 |
TATAGTA | 75 | 5.5750326E-5 | 28.0 | 2 |
TAGACAG | 100 | 4.406993E-7 | 27.999998 | 5 |
ATAACAC | 50 | 0.0072023305 | 27.999998 | 46 |
TATAAGA | 50 | 0.0072023305 | 27.999998 | 3 |
AACAGCG | 50 | 0.0072023305 | 27.999998 | 7 |
TTCTCAA | 50 | 0.0072023305 | 27.999998 | 2 |
GTACCGA | 65 | 7.9460815E-4 | 26.923079 | 6 |
TTGTACC | 65 | 7.9460815E-4 | 26.923079 | 4 |