FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308742
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC8770.284055943149944No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC8060.2610593958709861No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC7480.24227348400930226No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC5390.17457942230082074No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC4830.15644130050333288Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT4690.15190677005396092No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4340.140570443930531Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAA150.002223359270.01
TATATAC200.006947245452.53
GTCTAAT200.006947245452.51
CGTAATT200.006947245452.512
CCATACA400.002416702235.01
GTATAGT601.2136716E-535.01
CGAGTGC652.0990688E-532.30769370
TATGTGA552.988422E-431.818186
AATACGC450.004303628631.11110921
TTAGACA703.4826637E-530.04
TATATGT604.9759523E-429.1666664
GTCCTAA755.5750326E-528.01
TATAGTA755.5750326E-528.02
TAGACAG1004.406993E-727.9999985
ATAACAC500.007202330527.99999846
TATAAGA500.007202330527.9999983
AACAGCG500.007202330527.9999987
TTCTCAA500.007202330527.9999982
GTACCGA657.9460815E-426.9230796
TTGTACC657.9460815E-426.9230794