FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423984
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAGCTGCATTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACAC10800.25472659345635684No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA6770.1596758368240311No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC6050.14269406392694062No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5990.14127891618551644No Hit
GTATTTAGCTTTAGATGAAATTTACCACCCATTTAGAGCTGCATTCCCAA5540.1306653081248349No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5220.1231178535039058No Hit
GTCTAAGTGCCCTGGAACGGGCCATCGGAGAGGGTGAGAATCCCGTCTGG5040.11887241027963319No Hit
GGCACGGACACCCCATCCCAGACGGGATTCTCACCCTCTCCGATGGCCCG4870.11486282501226462No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCACGGACACC4850.11439110909845654No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTC200.00694967452.498768
TATATGT400.00241659835.0033044
TACTATG400.00241659835.0033042
ACTGCTC652.1012467E-532.306938
ACTAATA652.1012467E-532.3069350
TTAACGC450.00430592131.11037842
CAGAATT802.461944E-630.62789219
GTCTAGG1202.2882887E-929.1728611
TAGGGGT604.9763004E-429.169424
GGACTAA755.5718065E-528.0059451
TCGGTAA808.659399E-526.2493825
GATTGTT959.381316E-625.78886638
GCAGAAT1101.0135354E-625.4569518
CTCGTAT700.001225446224.9994139
TACGGAC700.001225446224.9994113
GTATGCC700.001225446224.9994142
CGAATTA700.001225446224.9994148
ACTCACA700.001225446224.9994124
TATCATA851.3062796E-424.7082146
CCACTAA851.3073263E-424.705348