FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491546
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATCCCCATCACGATGAAATTTCAAAGATTACCCGGACCTGTCGGC14070.28623974155012955No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC11280.22948004866279048No Hit
ATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTC10300.20954295223641325No Hit
GTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCCCT10020.2038466389717341No Hit
GGTGTGCACCGGTCGTCTCGTCCCTTCTGCCGGCGATGCGCTCCTGGCCT9700.19733656666924357No Hit
GTCATAATCCAGCGCACGGTAGCTTCGCGCCACTGGCTTTTCAACCAAGC9680.19692968715033793No Hit
GACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATTCCTCGTT9110.1853336208615267No Hit
GAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTTACAAGGATTCCC8490.17272035577545133No Hit
ATCAGATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGG8190.16661716299186646No Hit
ACACTGATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAA8040.16356556660007404No Hit
CCCTAACGTTGCCGTCAACCGCCGCGTCCCGGTTCAGGAATTTTAACCCG7520.15298669910852697No Hit
ACCTATACCCGGCCGTCGGGGCAAGCGCCAGGCCCCGATGAGTAGGAGGG7440.15135918103290436No Hit
GGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGGAATT7430.15115574127345152No Hit
CTTCCGATCTGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTT6980.14200095209807423No Hit
GTTCTGGGCCGCACGCGCGCTACACTGATGTATTCAACGAGTTTATAGCC6700.13630463883339505No Hit
GATGTATTCAACGAGTTTATAGCCTTGGCCGACAGGTCCGGGTAATCTTT6250.12714984965801776No Hit
CCTATAAGCAACATCCGCCGATCCCTGGTCGGCATCGTTTATGGTTGAGA6180.12572577134184795No Hit
CTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGT6080.12369137374731967No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC5900.12002945807716876No Hit
TCATTACTCCGATCCCGAAGGCCAACGTAATAGGACCGAAATCCTATAAT5760.11718130144482918No Hit
CATCAGTGTAGCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACC5630.1145365845719424No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT5460.11107810866124433No Hit
GTATCTGATCGTCTTCGAGCCCCCAACTTTCGTTCTTGATTAATGAAAAC5200.10578867491547077No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC5150.10477147611820663No Hit
ACGTAATAGGACCGAAATCCTATAATGTTATCCCATGCTAATGTATCCAG5130.10436459659930097No Hit
AATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTCAAAGT5060.1029405182831312No Hit
ACCATACTCCCCCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGG5040.10253363876422553No Hit
CCTATAATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGA5040.10253363876422553No Hit
ATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAGCACTCTAATTTCTTC4980.10131300020750857No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTAG252.3825894E-456.0337141
AGCCTGG252.3897889E-455.9995111
GTATATA200.006933158352.531611
GCATATG200.006949920752.49954625
GCGGGAC352.0858239E-549.9995714
GCCCACT651.0006261E-843.07654663
TGGGGAC1100.041.3632745
GTACTGG953.6379788E-1240.5507131
GTCTAGG350.001251358240.0240821
GTATAAT350.001251358240.0240821
AGGTATA350.001255121339.9996536
CTGTGGC350.001255121339.9996539
CTAGCCT350.001255121339.9996539
TAATCCG350.001255121339.99965310
CTATAGG459.161669E-538.91231
TCTCTAG556.6637986E-638.2048031
CCGGGAT556.691789E-638.18148414
TGTCCCG556.691789E-638.18148410
TCATCTA2000.036.7496825
ATGTAGT400.00241096135.0210721
Produced by FastQC (version 0.11.2)