Basic Statistics
Measure | Value |
---|---|
Filename | H3W35AFXY_n01_adr3wtzt14s12.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 422824 |
Sequences flagged as poor quality | 0 |
Sequence length | 76 |
%GC | 55 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC | 2466 | 0.583221387622273 | No Hit |
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC | 2329 | 0.5508201994210358 | No Hit |
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC | 2287 | 0.5408869884396345 | No Hit |
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC | 1782 | 0.42145195163945287 | No Hit |
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC | 842 | 0.1991372296747583 | Illumina Multiplexing PCR Primer 2.01 (95% over 22bp) |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA | 794 | 0.18778498855315687 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC | 616 | 0.14568709439388491 | No Hit |
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG | 526 | 0.12440164229088227 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTATAGG | 20 | 0.0069426545 | 52.512154 | 1 |
ATACATT | 30 | 5.875312E-4 | 46.66643 | 6 |
TAGGACT | 95 | 0.0 | 44.2103 | 4 |
CTATACT | 50 | 3.4757722E-6 | 41.999786 | 4 |
GGCATAG | 35 | 0.0012548999 | 39.999798 | 5 |
TATAATA | 45 | 9.185903E-5 | 38.89329 | 2 |
CTAGGAC | 110 | 0.0 | 38.181625 | 3 |
GAATCAG | 95 | 1.8735591E-10 | 36.84192 | 35 |
GGTTGCA | 105 | 1.4551915E-11 | 36.66648 | 16 |
AGGACTA | 110 | 2.3646862E-11 | 34.999825 | 5 |
CGGGATA | 50 | 1.7087096E-4 | 34.999825 | 15 |
AGATCTA | 40 | 0.00241777 | 34.99982 | 68 |
CGATTCG | 40 | 0.00241777 | 34.99982 | 14 |
ACATGTT | 40 | 0.00241777 | 34.99982 | 33 |
ACTGGTC | 65 | 2.1009675E-5 | 32.30753 | 8 |
ATGGTTG | 120 | 6.548362E-11 | 32.08317 | 14 |
ACTATAC | 55 | 2.9903676E-4 | 31.81802 | 3 |
TACGTCT | 55 | 2.9903676E-4 | 31.81802 | 4 |
TCGGGGG | 45 | 0.0043055178 | 31.110954 | 33 |
TATATCC | 45 | 0.0043055178 | 31.110954 | 4 |