FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences422824
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC24660.583221387622273No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC23290.5508201994210358No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC22870.5408869884396345No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC17820.42145195163945287No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC8420.1991372296747583Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA7940.18778498855315687Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTC6160.14568709439388491No Hit
GTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACG5260.12440164229088227No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG200.006942654552.5121541
ATACATT305.875312E-446.666436
TAGGACT950.044.21034
CTATACT503.4757722E-641.9997864
GGCATAG350.001254899939.9997985
TATAATA459.185903E-538.893292
CTAGGAC1100.038.1816253
GAATCAG951.8735591E-1036.8419235
GGTTGCA1051.4551915E-1136.6664816
AGGACTA1102.3646862E-1134.9998255
CGGGATA501.7087096E-434.99982515
AGATCTA400.0024177734.9998268
CGATTCG400.0024177734.9998214
ACATGTT400.0024177734.9998233
ACTGGTC652.1009675E-532.307538
ATGGTTG1206.548362E-1132.0831714
ACTATAC552.9903676E-431.818023
TACGTCT552.9903676E-431.818024
TCGGGGG450.004305517831.11095433
TATATCC450.004305517831.1109544