FastQCFastQC Report
Thu 26 Jul 2018
H3W35AFXY_n01_adr3wtzt14s11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3W35AFXY_n01_adr3wtzt14s11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410456
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC8730.21269027618063813No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC8720.21244664470734986No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT8710.21220301323406163No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8270.20148322840937882No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC6450.1571423002709182No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA5810.1415498859804705No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT5650.13765178240785858No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG5440.13253552146880543No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC5410.1318046270489407Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA5280.12863741789619348No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT5240.12766289200304054No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC4870.11864852749137544No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT4840.11791763307151071No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA4800.11694310717835772Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC4370.10646695382696318No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA4250.10354337614750424No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGAC150.0022239969.9991463
TTAACTC200.006945857352.50575345
TTTATAA350.001254901239.99951618
GTATGCC601.2137327E-535.00383842
GATACAC400.002417772534.9995733
TAGTCAT400.002417772534.99957353
ATATAAG751.4888847E-632.6662672
TAAGACA652.1009208E-532.30734
TGTAGGA450.00430552331.1107312
GGTCCAA1850.030.26990111
GTCTTAC2150.029.3019681
CATAGTG500.00720548127.9996618
TCTTTTT3450.027.3909713
CGTTTCT2450.027.14252717
AATCGAA2600.026.9260340
TCTTAGC657.951137E-426.922753
TAAGAAT657.951137E-426.9227530
GAGATAT657.951137E-426.9227520
CGTAAAT1850.026.48616423
GAATCGA2650.026.4179939
Produced by FastQC (version 0.11.2)