FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences969400
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38850.40076335877862596No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGGT6600.028.5877917
GATCTGG27450.026.603256
CGATCTG77150.026.4036165
TAACGTG808.768194E-526.2054775
CGATCTT33050.025.7958075
AGGTGTA8650.025.12962370
TAGGACA700.001238434524.9575964
GATCTTG11000.024.4584456
TGTACTT1001.4166035E-524.4584455
ATCTAGC4950.023.9996267
GATCTTT11350.023.704226
CCGATCT206800.023.5190334
ATCTGGG11600.023.4945667
ATCTAGG5250.023.2937567
AATAGTG750.001850748623.2937565
TTCCGAT210950.023.2867952
TCCGATC212500.023.0840553
ATCTTGC5100.022.6086467
ATCTGGC8600.022.3457557
TCTGACC3000.022.1290688
Produced by FastQC (version 0.11.2)