FastQCFastQC Report
Fri 3 Aug 2018
H3VNWAFXY_n02_adr3wtzt14s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3VNWAFXY_n02_adr3wtzt14s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699888
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34090.48707793246919506No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC10120.14459456370162083No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC9250.13216400338339848No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC8950.12787760327366665No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC8030.11473264293715565No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA7120.10173056260430241Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATGA200.007001333452.4030271
ATAAATT350.001262995239.95005816
ATAGCAT400.002440493834.935353
GTACTAC605.035527E-429.1127931
CATAAGC500.007272852627.948283
AGTTAAG657.877447E-426.96802763
TTCCGTA658.0048555E-426.89407222
TTTCGGA1950.026.8733461
ATACTGA700.001238496224.9559576
TACAATA851.3236917E-424.6623578
AACAGTG1202.1993546E-623.2922297
TATCAAG750.001851765223.2902322
TACGTCT1052.0677593E-523.2902324
GTCCTAA1703.8307917E-922.6052251
ACAATAT952.810848E-422.068219
ATACAGC952.812477E-422.066326
CTTTTTT4600.022.028239
ATGTGTA800.002661035421.88143537
ACAGTGC1604.992762E-821.8364648
CTGTGTG1454.675494E-721.6877239
Produced by FastQC (version 0.11.2)